Holmes was joined onstage by the Top Gun star as she sang the showtune Whatever Lola Wants, Lola Gets at Los Angeles' A Fine Romance Benefit for The Motion Picture and Television Fund on Saturday.
Dressed in a sexy black dress and heels, Holmes sashayed seductively towards her husband, who she pushed and pulled across the stage as she sang her big number.
Cruise played up his part and even allowed his wife to shove him onto the floor.
The crowd, which included Catherine Zeta-Jones, James Marsden and the event's host Hugh Jackman, cheered with delight as the couple shared a steamy kiss for the finale.
